Comparison of Stool Examination with the Formol-ether Sedimentation Method and the Kato-Katz Method in Detecting Soil-transmitted Helminth



  • Rilianda Abelira Fakultas Kedokteran Universitas Lampung
  • Hanna Mutiara Universitas Lampung



Kata kunci : STH, Metode Sedimentasi Formol -ether, Metode Kato-Katz.


Soil-transmitted Helminth (STH) is the most common intestinal parasitic infection that contribute to the global disease burden. Various health problems may occur due to this infection. STH infections can be diagnosed by microscopic examination of the stool. WHO recommends the Kato-Katz method as the gold standard for detecting STH, but this method is less sensitive to mild infections. Another method recommended for mild infections is the formol-ether concentration (FEC) method. Some studies show different results for the comparison of these two methods. This study aims to compare the result of the stool examination between FEC method and the Kato-Katz method in detecting STH. Evaluation of examination methods is important in the search for accurate diagnostic techniques.This study used stored stool samples. The study design was descriptive analytic with cross sectional approach. The sampling technique used is consecutive random sampling. Data were analyse based on STH frequency with McNemar's comparative statistical test. The microscopic picture of the FEC method is clearer than the microscopic picture of Kato-Katz method. The results of stool examination using the FEC method and the Kato-Katz method obtained positive results of 6 samples (20%) and negative samples of 24 samples (80%), same result for both method. The type of worm eggs found was Ascaris lumbricoides with 6 samples (100%). Hookworms (A.duodenale and N. americanus) found by FEC method was 2 samples (33.3%) of 6 positive samples. McNemar test results obtained p value of 1,000 (> α 0.05).There is no statistically significant difference from the results of the comparison of stool examination with the FEC method and the Kato-Katz method using McNemar test.


CDC, Soil-transmitted Helminths. [internet]. [disitasi tanggal 29 Januari 2020]. Tersedia dari:

Clarke NE, Clements ACA, Doi SA, et al. Differential effect of mass deworming and targeted deworming for soil-transmitted helminth control in children: a systematic review and meta-analysis. Lancet. 2017;389(10066):287-297.

Goodman D, Haji HJ, Bickle QD, et al. A comparison of methods for detecting the eggs of Ascaris, Trichuris, and hookworm in infant stool, and the epidemiology of infection in Zanzibari infants. Am J Trop Med Hyg. 2007;76(4):725-731.

Sanchez AL, Gabrie JA, Usuanlele MT, Rueda MM, Canales M, Gyorkos TW. Soil-Transmitted Helminth Infections and Nutritional Status in School-age Children from Rural Communities in Honduras. PLoS Negl Trop Dis. 2013;7(8).

Harhay MO, Horton J, Olliaro PL. Epidemiology and control of human gastrointestinal parasites in children. Expert Rev Anti Infect Ther. 2010;8(2):219-234.

Chessbrough M. District Laboratory Practice in Tropical Countries. Part 1. Vol 94.; 2000.

Prevention CC for DC and. CDC - Soil-Transmitted Helminths. Published online 2022.

Ridley JW. Laboratory Procedures for Identifying Parasitic Organisms and Their Ova.; 2012.

Endris M, Tekeste Z, Lemma W, Kassu A. Comparison of the Kato-Katz, Wet Mount andFormol-Ether Concentration Diagnostic Techniques for Intestinal Helminth Infections in Ethiopia. ISRN Parasitol. 2013;2013:1-5.

Knopp S, Mgeni AF, Khamis IS, et al. Diagnosis of soil-transmitted helminths in the era of preventive chemotherapy: Effect of multiple stool sampling and use of different diagnostic techniques. PLoS Negl Trop Dis. 2008;2(11).

Lim MD, Brooker SJ, Belizario VY, et al. Diagnostic tools for soil-transmitted helminths control and elimination programs: A pathway for diagnostic product development. PLoS Negl Trop Dis. 2018;12(3):1-18.

Taye S. Comparison of Kato-Katz and Formol-Ether Concentration Methods for the Diagnosis of Intestinal Helminthic Infections among School Children of Wonji Shoa Town, Eastern Ethiopia: A School Based Cross-Sectional Study. Am J Heal Res. 2014;2(5):271.

Fitriani CL, Panggabean M, Pasaribu AP. The accuracy of formol-ether concentration in diagnosing soiltransmitted helminths in elementary school 27 Peusangan in Bireuen. IOP Conf Ser Earth Environ Sci. 2018;125(1).

Singh Saharan G, Mehta N, Meena PD. The Disease. Alternaria Dis Crucif Biol Ecol Dis Manag. Published online 2016:17-51.

Garcia LS, Arrowood M, Kokoskin E, et al. Laboratory diagnosis of parasites from the gastrointestinal tract. Clin Microbiol Rev. 2018;31(1):1-81.



How to Cite

Abelira, R., & Mutiara, H. (2023). Comparison of Stool Examination with the Formol-ether Sedimentation Method and the Kato-Katz Method in Detecting Soil-transmitted Helminth: . Medical Profession Journal of Lampung, 13(4), 463-471.




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