Inhibition Zone Test of Honey Pineapple Peel Extract (Ananas comosus [L] Merr.) Againts Methicillin Resistant Staphylococcus aureus


  • Imraatul Husniah Universitas Lampung
  • Nurma Retno
  • Tri Umiana Soleha



antibacterial activity, honey pineapple peel, MRSA


MRSA infection is a global health problem which needed to be answered by developing a synthetic or natural antibacterial as an effort to overcome the problem. The honey pineapple peel contains potentially antibacterial compounds such as bromelain and flavonoid. The purpose of this study is to determine the antibacterial activity of honey pineapple peel extract (Ananas comosus [L] Merr.) against methicillin resistant Staphylococcus aureus (MRSA). This study was a true experimental study, with a post-test control group design. This study examined antibacterial activity of honey pineapple peel extract at concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.56% against MRSA bacteria by measuring inhibition zone with well method. The results of this study show that honey pineapple peel extract has antibacterial activity against MRSA at concentration 100% and 50% with the average diameter of inhibition zone is 19.35 mm and 16.97 mm. For the conclusion, there is antibacterial activity of honey pineapple peel extract (Ananas comosus [L] Merr.) against methicillin resistant Staphylococcus aureus (MRSA).


Liu Q, Jing W, Liu M, Liu J. Health disparity and mortality trends of infectious diseases in BRICS from 1990 to 2019. Journal of Global Health. 2022. 12; 04028: 1-11.

Setiabudy, R. Farmakologi dan Terapi Edisi 5. Dalam S. S. Gunawan editor (penyunting). Jakarta: Badan Penerbit Fakultas Kedokteran Universitas Indonesia; 2012

Asharina I. Resistensi Antibiotik Indonesia-Tak Usah Dulu Bermain Undang-Undang. Working Paper. Bandung: Institut Teknologi Bandung; 2017. doi:

Tacconeli et al. Discovery, Research, and Development of New Antibiotics: the WHO priority list of Antibiotic-Resistant Bacteria and Tuberculosis. Lancet Infection Disease. 2017. doi:

Lee et al. 2018. Methicillin-resistant Staphylococcus aureus. Nature Reviews. 4(18033):1-4.

Hassoun A, Lienden PK, Friedman B. 2017. Incidence, Prevalence, and Management of MRSA Bacteremia Across Patient Populations—a Review of Recent Developments in MRSA Management and Treatment. Critical Care. 21(211):1-10. doi:

WHO. 2018. Antibiotic Resistance. [Fact Sheets]

Firățoiu AR, Chereji AI, Chiurdu IA, Marcuta A. 2016. Study on The Production and Marketing of Pineapples Worldwide. Conference Paper of 37th IBIMA Conference: 30-31 May 2021, Cordoba, Spain. Available from

Kementrian Pertanian RI. 2016. Outlook Nenas. Available from

Punbasayakul N, Samart K, Sudmee W. Antimicrobial Activity of Pineapple Peel Extract. Proceeding of Innovation of Functional Foods in Asia Conference; 2018 April 24; Phayao. Thailand.

Xie Y, Yang W, Chen X. Antibacterial Activities of Flavonoids: Structure-Activity Relationship and Mechanism. Curr Med Chem. 2015. 22;1 :1-10.

Amini A, Setiasih S, Handayani S, Hudiyono S, Saepudin E. 2018. Potential Antibacteial Activity of Partial Purified Bromelain from Pineapple Core Using Acetone and Ammonium Suphate Againts Dental Caries-Causing Bacteria. AIP Conference Proceedings 2023. Universitas Indonesia.

Omorotionmwan FO, Ogwu HI, Ogwu MC. Antibacterial Characteristics and Bacteria Composition of Pineapple (Ananas comosus [Linn.] Merr.) Pell and Pulp. Food and Health. 2019 5;1 :1-11.

Mohapatra A, Rao VM, Ranjan M. Comparative Study of The Increase Production and Characteriation of Bromelain From the Peel, Pulp & Stem Pineapples. IJOART. 2013 2; 8 : 249-79.

Manaroinsong A, Abidjulu J, Siagian KV. Uji Daya Hambat Ekstrak Kulit Nanas (Ananas comosus L) terhadap Bakteri Staphylococcus aureus Secara In Vitro. Jurnal Ilmiah Farmasi UNSRAT. 2015. 4; 4 :27-31.

CLSI. Performance standards for antimicrobial susceptibility testing-CLSI approved standard M100-S28. Wayne: Clinical and Laboratory Standards Institute. 2018.

Rini ARS, Supartono, Wijayati N. 2017. Hand Sanitizer Ekstrak Kulit Nanas sebagai Antibakteri Staphylococcus aureus dan Escherichia coli. Indonesian Journal of Chemical Science. 6(1):61-66.

Ali AA, Milala MA, Gulani LA. 2015. Antimicrobial Effects of Crude Bromelain Extracts from Pineapple Fruit (Ananas comosus (Linn) Merr.). Advance in Biochemistry. 3(1):1-4.

Eumkeb G, Siriwong S, Phitakim S, Rojtinnakorn N, Sakdarat S. 2011. Synergistic Activity And Mode of Action of Flavonoid Isolated from Smaller Galagal and Amoxicillin Combination Against Amoxicillin-Resistant Escherichia coli. J Appl Microbiol. 112(1):55-64.



How to Cite

Husniah, I. ., Retno, N., & Soleha, T. U. (2023). Inhibition Zone Test of Honey Pineapple Peel Extract (Ananas comosus [L] Merr.) Againts Methicillin Resistant Staphylococcus aureus. Medical Profession Journal of Lampung, 13(4), 516-520.




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