Molecular Methods for Detecting Microbes Causing Pathogenic Diseases


  • Syazili Mustofa Universitas Lampung
  • Putri Adilla Faculty of Medicine (Lampung University)



Pathogens, Infections, Microbes, Molecular Methods


Pathogenic diseases are diseases that are an important problem for public health, especially in developing countries like Indonesia. This disease occurs when disease-causing microorganisms infect the host's body tissue and then multiply and react to the organism being attacked because of the toxins they produce. Several types of disease pathogens can be caused by viruses, bacteria, fungi and parasites. Detection of pathogenic bacteria as a source of infection still relies on isolation methods in pure media culture and microscopic analysis followed by biochemical reactions. However, when using existing techniques, it is difficult to isolate some bacteria in pure culture media, making it a challenge to develop other methods to identify the source of the infection. So far, identification and detection of pathogenic bacteria has only been carried out by observation based on clinical symptoms and history of disease, as well as morphological and physiological characteristics. However, this method is less efficient so a technique is needed that can detect pathogenic bacteria quickly and accurately. There are several types of methods that can be used to identify pathogenic bacteria, namely, methods that target nucleic acids, immunology-based methods, and biosensor-based methods.


Joegijantoro R. Penyakit Infeksi. Edisi 1. Intimedia. 2019.

Ali MT, Mahmud S, Mohsin M, Mian AU, Islam A, Ahmed FF. Knowledge, Attitude, and Practices Toward Food Safety Among Students In Bangladesh: A Cross-Sectional Web-Based Study. Heliyon. 2023;9(4):1-16.

Khairi, Amalia N, Nursofiah S, Rukminiati Y, Mursinah. Akankah Perkembangan Metode Deteksi Molekuler Era 4.0 Mampu Menggantikan Pemeriksaan Laboratorium Bakteri Secara Konvensional. Seminar Nasional Riset Kesehatan. 2020;15:380-385.

Muh A, Sukenda, Harris E, Sukadi MF, Yuhana, M. Identifikasi Molekuler Bakteri Patogen dan Desain Primer PCR.Jurnal Budidaya Perairan. 2013;1(3):43-50.

Soedarto S. Mikrobiologi Kedokteran. Edisi 1. Sagung Seto.2015

Khamesipour F, Doosti A, Taheri H. Molecular Detection Of Brucella Spp In The Semen, Testis and Blood Samples Of Cattle And Sheep. J Pure Appl Microbiol. 2013; 7:495-500.

Noor SM, Sudarmono PP, Kusumawati A, Karuniawati A. Deteksi Brucelosis Pada Susu Sapi Dengan Uji Polymerase Chain Reaction (PCR). J Kedokteran Hewan. 2015; 9:64-66.

Aydin Suleyman. 2015.A Short History, Principles, and Types of ELISA, and our Laboratory Experience with Peptide/Protein Analyses Using ELISA. Peptides.2015; (72):4-15.

Chao J, Zhu D, Zhang Y, Wang L, Fan C. DNA Nanotechnology-Enabled Biosensors. BiosensBioelectron. 2016;76:68-79.

Srinivasan B, Tung S. Development and Applications of Portable Biosensors. J Lab Autom. 2015;20:365-389.

Selvarajan S, Alluri NR., Chandrasekhar A, Kim SJ. Unconventional Active Biosensor Made Of Piezoelectric BaTiO3 Nanoparticles For Biomolecule Detection. Sens. Actuators B Chem. 2017;253:1180-1187.



How to Cite

Mustofa, S., & Putri Adilla. (2024). Molecular Methods for Detecting Microbes Causing Pathogenic Diseases. Medical Profession Journal of Lampung, 14(2), 351-354.




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